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采用分子生物学多聚酶链反应（ＰＣＲ）方法，对女性下生殖道疣状赘生物６１６例妇女（团块型３０７例，丘疹型３０９例），进行人乳头瘤状病毒ＨＰＶ＿（６，１１，１６，１８，３３，３５）ＤＮＡ等４８个亚型检测，并与以免疫组织化学ＡＢＣ方法检测ＨＰＶ－Ａｇ（衣壳抗原）及电镜、光镜、阴道镜等方法进行比较。结果：ＰＣＲ方法的灵敏度和特异性最强，团块型阳性率９７．９０％，丘疹型１．１０％，两型共存者均团块型阳性而丘疹型阴型。团块型ＨＰＶ－Ａｇ阳性率５３．５５％，诊断性空孢细胞７０．４９％、病毒颗粒５．８８％；丘疹型无ＨＰＶ－Ａｇ、无ＨＰＶ颗粒、无诊断性空泡细胞。提示：团块型为ＨＰＶｓ感染的尖锐湿疣，丘疹型为假性湿疣。
A total of 616 women with genital warty neoplasia (307 in mass and 309 in papulosis) were genotyped by molecular biological polymerase chain reaction (PCR) method. Human papillomavirus HPV6 (6,11,16 , 18,33,35) DNA were detected in 48 subtypes, and compared with immunohistochemical ABC method for detection of HPV-Ag (capsid antigen) and electron microscopy, light microscopy, colposcopy and other methods. Results: The sensitivity and specificity of PCR were the highest. The positive rate of mass was 97.90% and the papular type was 1.10%. Both types of coexisting were positive for lumps and papain - type. The positive rate of HPV-Ag was 53.55%, the diagnostic DNA was 70.49%, and the percentage of virus particles was 5.88%. The papulosis was HPV-Ag-free, with no HPV particles and no diagnostic vacuolar cells. Tip: The agglomerate is genital warts infected with HPVs and the papular type is pseudocyst.