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通过构建微宇宙湿地柱模拟气候升温的方法,采用高通量测序和核磁共振技术,分别研究了湿地土壤微生物群落和磷素形态对暖化作用的响应特征.结果表明,暖化作用导致了Firmicutes、Clostridia、Clostridiales、Clostridiaceae和Clostridium的相对丰度分别显著下降65%~98%、69%~87%、67%~87%、73%~97%和74%~93%,这表明暖化作用对不同分类水平上的物种Firmicutes到Clostridium具有显著的抑制效应.通过主坐标分析和聚类分析,不同湿地柱采样点的暖化组与对照组样本表现出显著的分离特征,揭示了暖化作用能够诱导微生物群落组成发生显著性变化.磷酸单酯和正磷酸盐是各湿地柱土壤主导的磷素形态,同时暖化作用导致了XX湿地柱采样点的磷酸单酯和正磷酸盐相对丰度分别显著升高275%和下降20%,JH湿地柱采样点的磷酸单酯和多聚磷酸盐相对丰度分别显著升高85%和下降49%,这表明不同磷素形态对暖化作用的响应具有土壤异质性特征.通过典型对应分析,揭示了暖化条件下微生物群落组成的显著变化对磷素形态具有显著的影响效应.
By constructing micro-universe wetland column to simulate climate warming, high-flux sequencing and nuclear magnetic resonance technique were used to study the response characteristics of soil microbial community and phosphorus form to warming in wetlands respectively. The results showed that the warming effect led to Firmicutes The relative abundances of Clostridia, Clostridiales, Clostridiaceae and Clostridium decreased by 65% -98%, 69% -87%, 67% -87%, 73% -97% and 74% -93%, respectively, Which significantly inhibited the species Firmicutes to Clostridium at different taxonomic levels.Through the main coordinate analysis and cluster analysis, the warming group and the control group in different wetland sampling sites showed significant separation characteristics, revealing the effects of warming Could induce significant changes in the microbial community composition.Phosphate monoesters and orthophosphates were the predominant phosphorus forms in the wetland soil column while the relative abundance of phosphate monoesters and orthophosphates in the XX wetland column sampling points were significantly different Increase of 275% and decrease of 20%, the relative abundance of phosphate monoesters and polyphosphates in JH wetland column increased by 85% and 49%, respectively, indicating that different phosphorus forms In response to warming action having heterogeneity of the soil. By the typical correspondence analysis, it revealed a significant change in the microbial community composition under warm conditions have a significant influence on the phosphorus fractions effects.