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本文研究睾丸随着温度升高的各个时期,在体内和体外间质细胞对人绒毛膜促性腺激素(hCG)刺激的反应。方法体内实验,用雄性 Wistar 鼠,60～70天龄,重350～500g。戊巴比妥钠麻醉后,阴囊浸于水中悬于盆上30′。水温按每组需要调节。体外实验:切下鼠左睾取100mg 组织培育在1ml Dul-beccos 培养基中,实验组培养基内加 1IU hCG。结果表明加温鼠的双侧睾丸39～41℃、30′,21天后无明显作用,但加热至41.5～43℃、30′,睾丸的重量明显减轻,伴有血中黄体生成素(LH)和卵泡刺激素(FSH)的水平显著升高。睾丸静脉血中睾酮浓度升高,但外周循环血中睾酮浓度无变化。hCG 造成最大刺激后,体内加热的睾丸分泌睾酮下降。hCG 造成最大刺激使体外加热的睾丸产生睾酮量超过正常2～3倍,但体外不加 hCG时,加热的睾丸产生睾酮量与对照组无差异。作者认为通过加热睾丸间质细胞产生的睾酮可能不如正常睾丸那样能有效地被分泌,这可能是因
This article studies the response of testicular cells to human chorionic gonadotropin (hCG) stimulation in vitro and in vivo at various stages of elevated temperature. Methods In vivo experiments, male Wistar rats, 60 to 70 days old, weighing 350 ~ 500g. After anesthesia with pentobarbital, the scrotum is drowned in water and suspended in a pot of 30 ’. Water temperature needs to be adjusted for each group. In vitro experiments: the left mouse testis was taken 100mg tissue culture in 1ml Dulbeccos medium, the experimental group medium plus 1IU hCG. The results showed that there was no significant effect on the testis of rats in 39 ~ 41 ℃, 30 ’and 21 days after heating, but the weight of testis was significantly reduced when heated to 41.5 ~ 43 ℃ and 30’, with the blood LH, And follicle stimulating hormone (FSH) levels were significantly higher. Testosterone testosterone concentration in blood increased, but peripheral blood testosterone concentration did not change. After hCG caused the greatest stimulation, testicular secretion of testosterone decreased in vivo. hCG caused the greatest stimulation of testicular testosterone in vitro produced more than 2 to 3 times the normal testosterone, but without hCG in vitro, the testosterone produced by heated testes and control group no difference. The authors believe that testosterone produced by heating testicular stromal cells may not be as efficiently secreted as normal testes, probably due to