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目的评估家蝇对溴氰菊酯抗性风险,预测抗性发展速率,为延长药剂使用寿命,更好地保护现有药剂的有效性,最大限度地发挥药剂潜在能力以及科学合理用药提供科学依据。方法采用室内抗性选育和生物测定方法获得用于抗性风险评估的品系;采用Tabashnik方法估算抗性现实遗传力(h2)并预测不同选择压力下抗性发展速率。结果经过21代的室内选育,家蝇对溴氰菊酯的抗性倍数上升了2197.55倍;家蝇对溴氰菊酯的抗性h2为0.1571,抗性风险较大;根据抗性发展规律(筛选前7代的抗性发展非常缓慢,从第8代开始迅速上升,筛选后期抗性发展基本稳定),计算不同阶段的h2分别为0.1016(F0~F7)、0.2140(F8~F17)和0.0250(F18~F21)。根据不同阶段的h2值(h2=0.1016,h2=0.2140,h2=0.0250),预测不同选择压力下(死亡率分别为50%、60%、70%、80%和90%),抗性上升10倍所需的代数分别为7.2~15.9、4.9~10.8和44.4~98.1代。结论家蝇对溴氰菊酯抗性风险较大,应根据防治对象的实际情况科学合理使用杀虫剂。
Objective To evaluate the resistance of housefly to deltamethrin and to predict the rate of resistance development. In order to provide a scientific basis for prolonging the service life of the drug, better protecting the effectiveness of the existing drug, maximizing the potency of the drug and scientifically rational drug use . Methods The lines used for resistance risk assessment were obtained by indoor resistance breeding and bioassay. The Tabashnik method was used to estimate the resistance heritability (h2) and to predict the rate of resistance development under different selection pressures. Results After 21 generations of indoor breeding, the resistance multiples of housefly to deltamethrin increased 2197.55 times; housefly resistance to deltamethrin h2 was 0.1571, the risk of resistance was high; according to the law of resistance development (The resistance of 7 generations before the screening showed a very slow development, rising rapidly from the 8th generation and stable development of the resistance at the late screening stage). The h2 values at different stages were 0.1016 (F0 ~ F7), 0.2140 (F8 ~ F17) and 0.0250 (F18 ~ F21). According to the h2 value of different stages (h2 = 0.1016, h2 = 0.2140, h2 = 0.0250), the resistance was predicted to increase by 10% at different selection pressures (mortality of 50%, 60%, 70%, 80% and 90% The required algebra is 7.2 ~ 15.9, 4.9 ~ 10.8 and 44.4 ~ 98.1 respectively. Conclusion Musca domestica has a higher risk of resistance to deltamethrin. The insecticide should be used scientifically and reasonably according to the actual situation of the control object.