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[目的]筛选Potter喷雾塔法测定丁醚脲对二斑叶螨敏感品系室内生物测定的最佳实验操作条件,为真实反映药剂对螨类的毒力提供依据。[方法]采用正交设计,设置不同的喷雾压力、喷雾剂量及沉降时间组合,综合各组合试验结果筛选出最佳操作条件。[结果]不同喷雾剂量下,沉积量都随喷雾压力和沉降时间的增加而减少;在相同剂量和沉降时间下,沉积量随喷雾压力增大而减少;在相同剂量和喷雾压力下,沉积量随沉降时间延长而减少;在相同喷雾压力和沉降时间下,沉积量与喷雾剂量成正比。方差分析结果表明,喷雾压力和喷雾剂量对沉积量有显著性影响,而沉降时间对其影响不显著。在相同的喷雾剂量下,丁醚脲对二斑叶螨的毒力随喷雾压力增大而减弱;在相同的喷雾压力下,毒力随喷雾剂量增加而增强。[结论]综合分析叶碟表面雾滴沉降与生物测定结果,推荐使用喷雾压力20 kPa,喷雾剂量1 mL;或者喷雾压力50 kPa,喷雾剂量1~1.5 mL作为二斑叶螨Potter喷雾塔法室内生物测定的最佳实验操作条件。
[Objective] The research aimed to screen the optimum experimental operating conditions for the determination of diafenthiuron susceptible strains by Potter spray tower method in order to provide the basis for the true reflection of the toxicity of pesticides to mites. [Method] Orthogonal design was used to set different combinations of spray pressure, spray dosage and settling time, and the optimum operating conditions were screened based on the results of each combination test. [Result] The deposition amount decreased with the increase of spray pressure and settling time under different spray dosages. The deposition amount decreased with the increase of spray pressure at the same dose and settling time. Under the same dose and spray pressure, the deposition amount With the settlement time to reduce and reduce; at the same spray pressure and settling time, the deposition amount is proportional to the spray dose. Analysis of variance showed that spray pressure and spray dosage had a significant effect on the sedimentation volume, but sedimentation time had no significant effect on it. Under the same spraying dose, the toxicity of diafenthiuron to Tetranychus urticae was weakened with the increase of spraying pressure. With the same spraying pressure, the virulence of diafenthiuron increased with the spraying dose. [Conclusion] Based on the comprehensive analysis of the results of droplet settlement and bioassay on leaf disk surface, it is recommended to use spray pressure of 20 kPa, spray volume of 1 mL, spray pressure of 50 kPa and spray volume of 1 to 1.5 mL as Potter spray tower method The best experimental operating conditions for bioassays.