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结扎切断 SD 大鼠左侧坐骨神经或前后根后,在一定的存活时间序列里,用 Olympus显徽镜的测光装置对经 EL—Badawi 氏改良法显色处理后的脊髓腰段前角乙酰胆硷酯酶(ACHE)进行了相对变化率(Xo/Xc—1)×100%的测定。结果发现坐骨神经切断术后,术侧脊髓前角 AChE10天后增高22.1%;术后25天时减少到-20.2%;60天时又再次增高22.8%,呈双峰夹低谷的变化曲线。切断前后根后,术侧脊髓前角 AChE10天后明显下降(-21.1%);提示外周神经受损部位不同,脊髓前角 AChE 发生变化的情况不同.根据分析,本文将双峰夹低谷曲线分为1.应激期;2.调整期;3.恢复期.外周神经受损后有关神经元将出现变性和再生现象.神经变性和再生过程中形态学方面的研究至今已有百余年的历史;神经递质及其代谢酶在神经系统中正常分布的研究也有了几十的年历史;但在神经变性和再生的过程中,神经递质及其代谢酶的动态变化的研究正在兴起。神经元受损后神经递质及其代谢酶的动态性变化,在一定程度上反映了神经细胞对伤害性刺激的反应。1987年 Malatova 在狗发现 AChE 在远侧残根中是先显著下降,后轻微下降;在近侧残根中和在前角中是先升后降.但在更长时间里的变化情况怎样?以及在大鼠脊髓前角中的变化情况又如何?这种变化与外周神经损伤部位的关系又怎样?目前尚无报导。为了探讨这些问题,本文应用组化方法进行染色,利用照相显微镜的曝光时间对AChE 的染色强度作了相对量的测定。
Ligation of the left sciatic nerve in rats after ligation or sciatic nerve root, in a certain survival time sequence, with Olympus magnifying mirror photometric device by EL-Badawi’s improved method of chromogenic treatment of spinal cord lumbar anterior horn acetylcholine The relative rate of change (Xo / Xc-1) × 100% of the alkaline esterase (ACHE) was determined. The results showed that after sciatic nerve transection, the anterior horn angle of the spinal cord on the side of the spinal cord increased by 22.1% after 10 days, decreased to -20.2% after 25 days and increased again by 22.8% at 60 days. After cutting the anterior and posterior roots, the anterior horn angle of the spinal cord on the operative side was significantly decreased (P <0.01) after 10 days (P <0.01), suggesting that the peripheral nerve injury sites were different and the changes of AChE in the spinal cord anterior horn were different.According to the analysis, 1. Stress period; 2. Adjustment period; 3. Recovery period. Peripheral nerve damage related neurons degeneration and regeneration will occur. Neurodegeneration and regeneration in the morphological aspects of the study has been more than a hundred years of history The research on the normal distribution of neurotransmitters and their metabolic enzymes in the nervous system also has several decades of history. However, in the process of neurodegeneration and regeneration, the research on the dynamic changes of neurotransmitters and their metabolic enzymes is on the rise. The dynamic changes of neurotransmitters and their metabolic enzymes after the neurons are damaged, to a certain extent, reflect the response of nerve cells to nociceptive stimuli. In 1987, Malatova found that AChE first significantly decreased in the distal remnant and then decreased slightly, while the proximal remnant root first increased and then decreased in the anterior horn, but what happened to the longer time? And in the rat spinal cord anterior horn changes and how? This change and the location of the peripheral nerve injury and how? There is no report yet. In order to explore these issues, we used histochemical staining methods to determine the relative intensity of AChE staining using the exposure time of a photomicroscope.