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用两种不同的方法提取橡胶叶片总DNA，结果表明：以SDS法提取的DNA更能满足对橡胶树进行大量RAPD分析的要求，其PCR扩增产物稳定，分别以不同的复性温度、变性和复性时间、循环数、DNA模板量和不同的Tag酶量进行比较实验．结果表明：以94℃变性1min和36℃复性1min、72℃延伸2min，DNA模板量为25～100ng，Tag酶量为1～1．5U．共进行45个循环为最适的PCR参数，能够得到清晰的扩增条带，且重复结果稳定一致。
Two different methods were used to extract total DNA from rubber leaves. The results showed that the DNA extracted by SDS method could meet the requirement of a large number of RAPD analysis of rubber trees. The products of PCR amplification were stable with different annealing temperature, denaturation and Refolding time, number of cycles, amount of DNA template and different amount of Tag enzyme. The results showed that the amount of DNA template was 25 ~ 100ng and the amount of Tag enzyme was 1 ~ 1.5U after denaturation at 94 ℃ for 1min and renaturation at 36 ℃ for 1min and extension at 72 ℃ for 2min. A total of 45 cycles for the most appropriate PCR parameters, can be a clear amplified bands, and the results were consistent and stable.