以肿瘤抗原MAGE-2为例,运用分子模拟方法对其4个优势候选HLA-A2限制性CTL表位进行初步鉴定,再通过多肽结合实验对鉴定结果进行验证.结果表明,4个候选肽中有3个与HLA-A2分子有较好的亲和力,进一步进行体外诱导CTL效应实验显示,3个高亲和力的表位肽可在体外有效诱导特异性CTL效应.利用计算机辅助鉴定CTL表位,克服了以往筛选肽要将肽逐一洗脱下来的繁琐实验过程,并且可直接观测到多肽复合物的结合模式,该技术在筛选大量优势CTL表位中具有较大的应用价值. Taking the tumor antigen MAGE-2 as an example, molecular HLA-A2 restricted CTL epitopes were preliminarily identified by molecular simulation method, and then the identification results were verified by peptide binding experiments.The results showed that among the four candidate peptides Three of them have good affinity with HLA-A2 molecules, and further CTL induction experiments in vitro showed that three high-affinity epitope peptides can effectively induce specific CTL effect in vitro.Using computer-assisted identification of CTL epitopes, In the past, the cumbersome experimental procedure that peptides were eluted one by one was used, and the binding mode of polypeptide complexes was directly observed. This technique has great value in screening a large number of dominant CTL epitopes.