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[目的]为揭示铬对玉米的伤害机制提供理论依据。[方法]通过测定不同浓度Cr6+胁迫下玉米幼苗根和上胚轴的生理生化指标研究Cr6+胁迫对玉米幼苗生理生化特性的影响。[结果]在20 mg/L Cr6+胁迫下,幼苗根部的Pro积累量达到峰值,比对照增加了1.60倍;在50 mg/L Cr6+胁迫下,上胚轴的Pro积累量达到峰值,比对照增加了1.48倍。在100 mg/L Cr6+胁迫下,根和上胚轴的质膜透性分别是对照的1.63和1.48倍,MDA含量分别是对照的1.51和1.37倍,SOD活性分别比对照下降了27.7%和22.9%;根部的POD活性比对照低9.9%,上胚轴的POD活性比对照高13.6%。在10 mg/L Cr6+胁迫下,根部POD活性达到峰值,比对照增加了49.2%;在50 mg/L Cr6+胁迫下,上胚轴POD活性达到峰值,比对照增加了57.9%。[结论]玉米幼苗对铬胁迫的耐受性是有限的。
[Objective] The research aimed to reveal the mechanism of chromium damage to maize. [Method] The effects of Cr6 + stress on physiological and biochemical characteristics of corn seedling were studied by measuring the physiological and biochemical indexes of maize seedling roots and hypocotyls under different Cr6 + stress. [Result] Under the condition of 20 mg / L Cr6 + stress, the accumulation of Pro in seedling roots reached the peak value, which was 1.60 times higher than that of the control. Under the treatment of 50 mg / L Cr6 +, the Pro accumulation in the hypocotyl reached its peak, 1.48 times. Under 100 mg / L Cr6 + stress, the membrane permeability of roots and hypocotyls were 1.63 and 1.48 times of the control respectively, the content of MDA was 1.51 and 1.37 times of the control respectively, and the SOD activity decreased by 27.7% and 22.9% respectively compared with the control %; Root activity of POD was 9.9% lower than that of the control and POD activity of the hypocotyl was 13.6% higher than that of the control. Under the stress of 10 mg / L Cr6 +, the activity of POD in roots reached a peak value 49.2% higher than that of the control. Under the stress of 50 mg / L Cr6 +, the activity of POD in the hypocotyl reached its peak, increasing 57.9% compared with the control. [Conclusion] The tolerance of maize seedlings to chromium stress was limited.