论文部分内容阅读
目的掌握深圳市从禽类市场外环境及人感病例中分离到的H7N9禽流感病毒神经氨酸酶(NA)基因的演化,以及病毒对神经氨酸酶抑制剂(NAI)的耐药情况,为人感染H7N9病例的临床救治方案提供参考。方法将深圳市11份人感染H7N9禽流感病例鼻咽拭子标本以及3份活禽市场的环境拭子样本,经分离培养后对其NA片段进行全长测序。并选取国内外的H7N9病毒株序列作为参考,运用Mega软件进行种系发生树的构建、耐药相关位点及糖基化位点的分析。结果对NA片段的序列分析发现,所有深圳株NA基因的蛋白抗原位点和糖基化位点都相对保守,并且未出现R294K耐药位点。但发现三株未出现H7N9特征性蛋白柄部氨基酸的缺失株。结论深圳市分离到的H7N9病毒株与中国其它省份的流行株高度同源,对NAI仍然敏感。蛋白柄部氨基酸变异提示还需要对其进行进一步的研究和监测。
Objective To understand the evolution of the neuraminidase (NA) gene of H7N9 bird flu virus isolated from the poultry market and the human environment and the virus resistance to neuraminidase inhibitor (NAI) Infection H7N9 cases of clinical treatment programs provide a reference. Methods Nasopharyngeal swab samples of H7N9 bird flu and three samples of live swabs from the live poultry market were obtained from 11 human in Shenzhen City, and their NA fragments were sequenced after being isolated and cultured. The sequence of H7N9 strain at home and abroad was selected as a reference, and phylogenetic tree construction, resistance-related sites and glycosylation sites were analyzed by Mega software. Results Sequence analysis of NA fragments showed that all the NA genes in Shenzhen strain were relatively conserved in protein and glycosylation sites, and no R294K resistance site was found. However, three deletion strains which did not show the amino acid residues of H7N9-specific proteins were found. Conclusion The H7N9 strain isolated in Shenzhen is highly homologous to the epidemic strains in other provinces of China and is still sensitive to NAI. Amino acid variations in protein stems suggest that further research and monitoring is needed.