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目的:探讨鼻咽癌患者外周血CD4+CD25h igh调节性T(Tr)细胞比例变化的特点及其临床意义。方法:采用流式细胞术检测54例初治鼻咽癌患者(鼻咽癌组)和15例健康者(对照组)外周血中CD3+、CD4+、CD8+及CD4+CD25h ighT细胞比例。结果:鼻咽癌患者CD4+T细胞比例、CD4+/CD8+比值均低于对照组(均P<0.05),但CD4+CD25h ighTr细胞比例明显高于对照组(P<0.01)。随疾病进展鼻咽癌患者外周血CD4+CD25h ighTr细胞比例升高[Ⅰ+Ⅱ期为(2.28±1.07)%,Ⅲ期为(3.65±1.21)%,Ⅳ期为(4.72±0.87)%],Ⅰ+Ⅱ期与Ⅲ、Ⅳ期比较,均P<0.01;Ⅲ期与Ⅳ期比较,P<0.01。结论:CD4+CD25h ighTr细胞可能是鼻咽癌患者免疫抑制的重要原因之一,与鼻咽癌免疫逃逸有关。外周血CD4+CD25h ighTr细胞比例可能成为鼻咽癌一种新的预后判断指标,去除这群细胞可有效诱导肿瘤免疫,为肿瘤治疗提供一种新的方法。
Objective: To investigate the characteristics and clinical significance of CD4 + CD25h high regulatory T (Tr) cells in peripheral blood of patients with nasopharyngeal carcinoma. Methods: Flow cytometry was used to detect the percentage of CD3 +, CD4 +, CD8 + and CD4 + CD25h high T cells in peripheral blood of 54 patients with nasopharyngeal carcinoma (nasopharyngeal carcinoma) and 15 healthy controls (control group). Results: The proportion of CD4 + T cells and the ratio of CD4 + / CD8 + in nasopharyngeal carcinoma patients were significantly lower than those in control group (all P <0.05), but the proportion of CD4 + CD25h high T cells was significantly higher than that in control group (P <0.01). The proportion of CD4 + CD25h high T cells in peripheral blood of patients with nasopharyngeal carcinoma increased with the progression of disease (2.28 ± 1.07% in stage Ⅰ + 3.65 ± 1.21% in stage Ⅲ and 4.72 ± 0.87% in stage Ⅳ) , P <0.01 in stage Ⅰ + Ⅱ and stage Ⅲ, Ⅳ, P <0.01 in stage Ⅲ and Ⅳ. Conclusion: CD4 + CD25h ighTr cells may be one of the important reasons for immunosuppression in patients with nasopharyngeal carcinoma, which is related to immune escape of nasopharyngeal carcinoma. The proportion of CD4 + CD25h high T cells in peripheral blood may become a new prognostic indicator for NPC. Removing these cells can effectively induce tumor immunity and provide a new method for tumor therapy.