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目的:建立高效液相色谱法同时测定维药驱虫斑鸠菊中圣草酚、紫铆查耳酮、紫铆素和异鼠李素含量的方法,为完善驱虫斑鸠菊质量标准提供依据。方法:以圣草酚、紫铆查耳酮、紫铆素和异鼠李素为对照品,采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈为流动相A,0.2%磷酸水溶液为流动相B,进行梯度洗脱,检测波长310 nm,流速1.0 m L·min-1,柱温25℃,测定驱虫斑鸠菊药材中这4个黄酮类成分的含量。结果:本法中圣草酚、紫铆查耳酮、紫铆素和异鼠李素的进样量分别在13.44~107.52、30.24~241.92、9.60~76.80、6.25~49.97 ng范围内与峰面积积分值呈良好线性关系,r分别为0.9998、0.9999、0.9991、0.9997,平均加样回收率(n=9)在99.70%~100.56%,RSD小于2.0%。结论:本法简单、快速、重现性好,可为评价驱虫斑鸠菊药材的质量提供依据。
OBJECTIVE: To establish a method for the simultaneous determination of eriodictyol, vitechauron, vinorelbine and isorhamnetin of Vernonia lupo by high performance liquid chromatography (HPLC) in order to provide basis for improving the quality standard of Vernonia lutea. Methods: Eriocatropin, rivetchalcone, rivastigmine and isorhamnetin were used as reference materials, and the mobile phase A was obtained with Agilent ZORBAX SB-C18 (4.6 mm × 250 mm, 5μm) 0.2% phosphoric acid solution was used as mobile phase B, gradient elution was carried out. The detection wavelength was 310 nm, the flow rate was 1.0 m L · min-1 and the column temperature was 25 ℃. Results: The injection rates of eriodictyol, rivetchalcone, rivastigmine and isorhamnetin in this method were respectively in the range of 13.44 ~ 107.52, 30.24 ~ 241.92, 9.60 ~ 76.80, 6.25 ~ 49.97 ng and peak area The integral values showed a good linear relationship, r were 0.9998,0.9999,0.9991,0.9997, the average recovery rate (n = 9) was 99.70% ~ 100.56%, RSD was less than 2.0%. Conclusion: This method is simple, rapid, reproducible, and can provide a basis for evaluating the quality of insect-resistant Vernonia.