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为创建结球甘蓝—大白菜异附加系,以大白菜二倍体(AA,2n=2x=20)与结球甘蓝四倍体(CCCC,2n=4x=36)杂交获得的8个异源三倍体(ACC,2n=3x=28)单株系为试材,进行游离小孢子培养研究,获得再生植株,并对其进行了染色体数目鉴定和性状调查。结果表明,异源三倍体(ACC)的小孢子诱导成胚的能力低于双亲;在NLN中添加15%的蔗糖和0.1mg·L-16-BA、0.2mg·L-12,4-D、0.05mg·L-1KT的培养基上小孢子胚胎发生最高为0.0250个.蕾-1,8个株系间有差异,平均为0.0100个·蕾-1;胚胎再生率只有33.3%。小孢子再生植株染色体数在14～27条之间,田间性状表现多样。
In order to create a new hybrid between cabbage and Chinese cabbage, 8 heterologous plants obtained by crossing Chinese cabbage diploid (AA, 2n = 2x = 20) with tetraploid cabbage (CCCC, 2n = 4x = 36) The triploid (ACC, 2n = 3x = 28) lines were used as test materials for the study of free microspore culture. The regenerated plants were obtained and their chromosome numbers were identified and traits investigated. The results showed that the microspores of allotriploid (ACC) had less ability to induce embryogenesis than their parents. The addition of 15% sucrose and 0.1 mg · L-16-BA, 0.2 mg · L-12,4- D and 0.05mg · L-1KT, the highest number of microspore embryogenesis was 0.0250, the difference between Lei-1 and 8 lines was about 0.0100 · L-1 and the embryo regeneration rate was only 33.3%. Microspore regeneration plants chromosome number between 14 ~ 27, field performance varied.