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构建了8个PLZF-RARα融合基因突变体.用“滞后”胶实验证实PLZF-RARα与PML-RARα一样,亦能以同二聚体的形式结合到维甲酸反应元件(RARE)上,且PLZF的POZ结构域介导PLZF-RARα同二聚体的形成和稳定.但两者与RARE的结合类型存在差异,在DR5G,PML-RARα的结合强度大于PLZF-RARα;而在DR5T,则是PLZF-RARα强于PML-RARα.进一步工作证实PLZF-RARα能与RXR形成异二聚体,并产生4种复合物.用免疫沉淀法发现PLZF-RARα亦能与PLZF形成异二聚体,而且也是通过POZ结构域介导PLZF-RARα和PLZF异二聚体的形成.同PML-RARα一样,PLZF-RARα对RARE的结合反应亦受维甲酸调控.
Eight PLZF-RARα fusion gene mutants were constructed. It was confirmed by “hysteresis” gelation that PLZF-RARα, like PML-RARα, can also bind to Retinoic Acid Response Element (RARE) in the form of homodimer and PLZF. The POZ domain mediates the formation and stabilization of the PLZF-RARα homodimer. However, the binding patterns of the two are different from those of RARE. In DR5G, the binding strength of PML-RARα is greater than that of PLZF-RARα; in DR5T, it is PLZF. -RARα is stronger than PML-RARα. Further work confirms that PLZF-RARα can heterodimerize with RXR and produce four complexes. PLZF-RARα can also form heterodimers with PLZF by immunoprecipitation. The POZ domain mediates the formation of PLZF-RARα and PLZF heterodimers. Like PML-RARα, the binding of PLZF-RARα to RARE is also regulated by retinoic acid.