论文部分内容阅读
[目的]观察益气清毒方联合环磷酰胺(CTX)的抗肿瘤效果并探讨其作用机制。[方法]水煎煮制备益气清毒方汤剂,建立H22荷瘤小鼠动物模型,接种后第2d开始给药,腹腔注射CTX及益气清毒方灌胃,第9d称重后处死小鼠,眼眶取血做血液学检查,并观察小鼠胸腺、脾脏及肿瘤瘤体生长情况。[结果]与CTX低剂量组相比,CTX+益气清毒方高、中剂量组小鼠胸腺指数明显增加,瘤重下降,白细胞及血小板计数无明显下降。[结论]益气清毒方能明显加强CTX的抗肿瘤作用,抑制H22荷瘤小鼠肿瘤生长,减轻CTX不良反应,提高免疫功能。
[Objective] To observe the antitumor effect of Yiqi Qingdu prescription combined with cyclophosphamide (CTX) and to explore its mechanism. [Method] Decoction of Yiqi qingdu decoction was prepared by decoction with water and the animal model of H22 tumor-bearing mice was established. The rats were administrated on the 2nd day after inoculation, intraperitoneally injected with CTX and Yiqi qingdu decoction, and were weighed and sacrificed on the 9th day Mice and orbital blood was taken for hematological examination and the growth of thymus, spleen and tumor in mice was observed. [Results] Compared with CTX low dose group, the thymus index of CTX + Yiqi Qingdu Formula high and middle dose groups increased obviously, the tumor weight decreased, the white blood cells and platelet count did not decrease obviously. [Conclusion] Yiqi Qingdu decoction can significantly enhance the anti-tumor effect of CTX, inhibit the tumor growth of H22 tumor-bearing mice, reduce the adverse reaction of CTX and improve the immune function.