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目的建立用HPLC同时测定复方益肝灵片中水飞蓟宾、五味子醇甲的含量。方法Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水,梯度洗脱为0→60 min(甲醇40%→75%,水60%→25%),检测波长:水飞蓟宾287 nm,五味子醇甲250 nm。结果水飞蓟宾和五味子醇甲分别在4.96~39.68μg.mL-1(r=0.999 8)和4.00~32.00μg.mL-1(r=0.999 8)内呈良好的线性关系,平均加样回收率分别为98.8%和99.5%,RSD分别为0.96%和0.59%。结论该方法简便、快速、重复性好,可用于复方益肝灵片的质量控制。
Objective To establish a simultaneous HPLC method for the determination of Silybin and Schizandrol A in compound Yiganling tablets. Method Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), mobile phase methanol-water, gradient elution 0 → 60 min (methanol 40% → 75%, water 60% → 25%), detection wavelength: water Feizabin 287 nm, Schizandrin 250 nm. Results Silybin and Schizandrin A were linear in the range of 4.96-39.68 μg.mL-1 (r=0.999 8) and 4.00-32.00 μg.mL-1 (r=0.999 8), respectively. The recovery rates were 98.8% and 99.5%, respectively, with RSDs of 0.96% and 0.59%, respectively. Conclusion The method is simple, rapid, and reproducible. It can be used to control the quality of compound Yiganling tablets.