【摘 要】
：
通过聚合酶链式反应(PCR)研究稀土离子对基因序列的体外诱变作用。以克隆在质粒上的乙酰化酶基因做模板进行PCR,在反应体系中加入不同浓度的稀土离子,并将PCR产物克隆测序。
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通过聚合酶链式反应(PCR)研究稀土离子对基因序列的体外诱变作用。以克隆在质粒上的乙酰化酶基因做模板进行PCR,在反应体系中加入不同浓度的稀土离子,并将PCR产物克隆测序。稀土离子对PCR有影响,当稀土离子的浓度达到1×10~(-3)mol/L时可以抑制PCR;将诱变后的乙酰化酶基因序列与原序列对比发现,稀土离子可以在体外通过PCR引起基因突变。突变是点突变即由T变为C和由G变为A的转换。
In vitro mutagenesis of gene sequences by rare earth ions was investigated by polymerase chain reaction (PCR). PCR was carried out using the template of the acetylase gene cloned on the plasmid. Different concentrations of rare earth ions were added to the reaction system, and the PCR products were cloned and sequenced. The effect of rare earth ions on PCR can be inhibited when the concentration of rare earth ions reaches 1 × 10 ~ (-3) mol / L. The comparison of the sequence of the mutated acetylase gene with the original sequence shows that the rare earth ions can be in vitro Gene mutation by PCR. Mutations are point mutations that change from T to C and from G to A.
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