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低温抽提Jurkat细胞膜去污剂抗性的膜成分(DIG),检测Src家族酪氨酸激酶(PTK)及衰变加速因子(DAF)的分布情况。共聚焦扫描显微技术检测Jurkat细胞静息与交联状态下DAF分子对去污剂Triton-X100的抗溶性,以探讨其在Jur-kat细胞免疫识别中的作用,结果显示静息状态CD3对去污剂无抗溶性,而DAF与Lck有抗溶性。单抗交联后CD3对去污剂抗性有所增加。静息状态DAF与Src家族PTK特异分布于膜微区中,交联CD3可诱导CD3与膜微区特异性聚集,DAF所在的膜微区可能促进T细胞的免疫识别和信号传递作用。
The membrane fraction (DIG) of Jurkat cell membrane detergent resistance was extracted at low temperature, and the distribution of Src family tyrosine kinase (PTK) and decay accelerating factor (DAF) was detected. Confocal scanning microscopy was used to detect the anti-soluble effect of DAF molecules on the detergent Triton-X100 in resting and cross-linked Jurkat cells to investigate its role in Jur-kat cellular immune recognition. The results showed that the quiescent CD3 pairs Detergents are non-soluble, while DAF and Lck are anti-soluble. Antibacterial resistance of CD3 increased after MAb cross-linking. Resting DAF and Src family PTK are located in the membrane microarray. Cross-linking of CD3 can induce specific aggregation of CD3 and membrane micro-domains. The membrane microarray where DAF resides may promote the immune recognition and signal transduction of T cells.