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研究已证实,PDGF是动脉平滑肌细胞迁移和增殖的主要因素.在动脉粥样硬化的初始阶段,PDGF主要来自动脉壁上粘附和聚集的血小板,但血小板本身并无合成蛋白质的能力.因此,本实验利用光敏生物素标记sis基因探针和PDGF—A链基因探针,采用Dot blot RNA—DNA杂交法,探测了体外培养豚鼠骨髓巨核细胞中sis基因和PDGF—A链基因的表达,并观察了IL—la,IL—6,TNFa,IFNr,GM—CSF和凝血酶(TB)对两种基因表达的影响.结果显示:1.骨髓巨核细胞中同时表达sis基因和PDGF—A链基因mRNA,且两者
Studies have shown that PDGF is a major factor in the migration and proliferation of arterial smooth muscle cells, which are mainly derived from platelets that attach and accumulate on the arterial wall during the initial stages of atherosclerosis, but platelets themselves do not have the ability to synthesize proteins Therefore, In this study, the biotin-labeled sis gene probe and PDGF-A chain gene probe were used to detect the expression of sis gene and PDGF-A gene in guinea pig bone marrow megakaryocytes by Dot blot RNA-DNA hybridization The effects of IL-la, IL-6, TNFa, IFNr, GM-CSF and thrombin (TB) on the expression of two genes were observed.The results showed that: 1.The gene of sis and PDGF-A were expressed in the bone marrow megakaryocytes simultaneously mRNA, and both