Grassland ecosystem is an important component of the terrestrial carbon cycle system. Clear comprehension of soil organic carbon(SOC) storage and potential of grasslands is very important for the effective management of grassland ecosystems. Grasslands in Inner Mongolia have undergone evident impacts from human activities and natural factors in recent decades. To explore the changes of carbon sequestration capacity of grasslands from 2000 to 2012, we carried out studies on the estimation of SOC storage and potential of grasslands in central and eastern Inner Mongolia, China based on field investigations and MODIS image data. First, we calculated vegetation cover using the dimidiate pixel model based on MODIS-EVI images. Following field investigations of aboveground biomass and plant height, we used a grassland quality evaluation model to get the grassland evaluation index, which is typically used to represent grassland quality. Second, a correlation regression model was established between grassland evaluation index and SOC density. Finally, by this regression model, we calculated the SOC storage and potential of the studied grasslands. Results indicated that SOC storage increased with fluctuations in the study area, and the annual changes varied among different sub-regions. The SOC storage of grasslands in 2012 increased by 0.51×1012 kg C compared to that in 2000. The average carbon sequestration rate was 0.04×1012 kg C/a. The slope of the values of SOC storage showed that SOC storage exhibited an overall increase since 2000, particularly for the grasslands of Hulun Buir city and Xilin Gol League, where the typical grassland type was mainly distributed. Taking the SOC storage under the best grassland quality between 2000 and 2012 as a reference, this study predicted that the SOC potential of grasslands in central and eastern Inner Mongolia in 2012 is 1.38×1012 kg C. This study will contribute to researches on related methods and fundamental database, as well as provide a reference for the protection of grassland ecosystems and the formulation of local policies on sustainable grassland development. Grassland ecosystem is an important component of the terrestrial carbon cycle system. Clear comprehension of soil organic carbon (SOC) storage and potential of grasslands is very important for the effective management of grassland ecosystems. Grasslands in Inner Mongolia have undergone visible impacts from human activities and To explore the changes of carbon sequestration capacity of grasslands from 2000 to 2012, we carried out studies on the estimation of SOC storage and potential of grasslands in central and eastern Inner Mongolia, China based on field investigations and MODIS image First, we calculated the vegetation cover using the dimidiate pixel model based on MODIS-EVI images. Following field investigations of aboveground biomass and plant height, we used a grassland quality evaluation model to get the grassland evaluation index, which is typically used to represent grassland quality. Second, a correlation regression model was established bet Finally, by this regression model, we calculated the SOC storage and potential of the studied grasslands. Results showed that SOC storage increased with fluctuations in the study area, and the annual variation varied among different sub-regions . The SOC storage of grasslands in 2012 increased by 0.51 × 1012 kg C compared to that in 2000. The average carbon sequestration rate was 0.04 × 1012 kg C / a. The slope of the values ​​of SOC storage showed that SOC storage showed an overall Taking the SOC storage under the best grassland quality between 2000 and 2012 as a reference, this study predicted that the SOC potential of grasslands in central and eastern Inner Mongolia in 2012 is 1.38 × 1012 kg C. This study will contribute to researches on related methods and fundamental database, as well as provide a reference for the protection of grassland ecosystems and the formulation of local policies on sustainable grassland development.