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为了揭示白菜(Brassica rapa ssp.chinensis)BrLOM2基因在叶发育中的调控机制,以白菜圆叶、裂叶近等基因系为材料,同源克隆得到BrLOM2基因,分析其时空表达特性及对激素的响应。结果显示,BrLOM2的ORF全长为549 bp,编码182个氨基酸,含有GRAS家族保守结构域,定位于细胞核,两种材料中的氨基酸序列在保守结构域存在两处突变。进化分析表明,BrLOM2属于十字花科LOM2分支,与大白菜(XP_009138823.1)亲缘关系最近。RT-PCR结果显示,BrLOM2在圆叶白菜中的表达量高于同期裂叶白菜,两种材料茎尖、第1片叶中表达量最高;裂叶中的表达量与叶缘裂刻数呈现同步增加的趋势,6-BA和GA_3分别抑制和促进叶片中的表达,说明BrLOM2低表达可能是裂叶表型产生的主要原因。
In order to reveal the regulatory mechanism of BrLOM2 gene in leaf development of Brassica rapa ssp.chinensis, the BrLOM2 gene was cloned by homologous cloning of cabbage leaves and cleaved leaves, and the spatial and temporal expression characteristics of BrLOM2 gene were analyzed. response. The results showed that the ORF of BrLOM2 was 549 bp in length, encoding a polypeptide of 182 amino acids. It contained the GRAS family of conserved domains and located in the nucleus. The two amino acid sequences of the two proteins had two mutations in the conserved domain. Phylogenetic analysis showed that BrLOM2 belonged to the LOM2 branch of the crucifer, with the closest relationship with Chinese cabbage (XP_009138823.1). The results of RT-PCR showed that the expression level of BrLOM2 in cabbage was higher than that of cabbage in the same period. The expression of BrLOM2 was the highest in leaves of the two materials, 6-BA and GA_3, respectively, inhibited and promoted the expression of the leaf, indicating that the low expression of BrLOM2 may be the main reason for the appearance of split-leaf phenotype.