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应用基因枪法对超级杂交水稻培矮-64S进行了转化。质粒载体pKC-3串联了三个抗虫基因,将其转化成熟胚诱导的愈伤组织,共获得33株转基因植株。分别对不同的基因进行了PCR分析,结果表明,三个抗虫基因均已整合到水稻基因组。
Transgenic rice Pei’ai-64S was transformed by particle bombardment. Three insect-resistant genes were cloned in pKC-3 vector and transformed into mature embryo-induced callus. A total of 33 transgenic plants were obtained. PCR analysis of different genes was carried out. The results showed that all the three insect-resistant genes were integrated into rice genome.