本试验以8份花生种质资源为试材,摸索适宜的DNA提取方法,并通过大量文献的调研初步筛选出4套反应体系,并使用不同引物对其进行筛选,最终确立了适合花生SSR-PCR反应体系为10μL,其中各组分终浓度如下:25 ng DNA,1×buffer,0.15μmol/L引物,200μmol/L d NTP,0.5UTaq酶。最终确立的扩增程序为:94℃预变性5 min,94℃变性1 min,55℃退火30 s,72℃延伸1 min,35个循环,72℃延伸5 min。 In this study, 8 peanut germplasm resources were used as experimental materials to explore suitable DNA extraction methods. Four sets of reaction systems were initially screened through extensive literature investigation and screened with different primers to finally establish a suitable SSR- The final concentration of each component was as follows: 25 ng DNA, 1 × buffer, 0.15 μmol / L primer, 200 μmol / L d NTP, 0.5 Uaq enzyme. The final amplification procedure was: denaturation at 94 ° C for 5 min, denaturation at 94 ° C for 1 min, annealing at 55 ° C for 30 s, extension at 72 ° C for 1 min, 35 cycles and extension at 72 ° C for 5 min.