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目的 :研究亚低温状态下 ,急性脑缺血时皮质内四种氨基酸和自由基含量的改变 ,探讨亚低温对缺血脑组织的保护机制。方法 :将 63只 Wistar大鼠随机分为 9组 ,假手术组 7只 ,缺血组 (手术后将其颞肌温度控制在 36.5± 0 .5℃ ) -缺血 3h组 ,缺血 3h分别再灌注 1 h、2 h、3h组各 7只 ;亚低温组 (手术后将其颞肌温度控制在 32 .5± 0 .5℃ )一缺血 3h,缺血 3h分别再灌注 1 h、2 h、3h组各 7只。动物模型参照 Zea longa的大脑中动脉线栓模型并加以改进。结果 :比较缺血组和低温组 ,可见低温组缺血皮质内的谷氨酸 (Glu)、天门冬安酸 (Asp)、丙二醛 (MDA)的产生明显减少 ,而超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-PX)的生成和释放明显增加 ,γ-氨基丁酸(GABA)的含量亦增加。结论 :亚低温状态下 4种氨基酸含量及自由基系统的改变是其保护缺血神经元的重要机制
OBJECTIVE: To study the changes of four amino acids and free radicals in cortex under mild hypothermia, and to explore the mechanism of hypothermia on ischemic brain. Methods: Sixty-three Wistar rats were randomly divided into 9 groups: sham operation group (7 rats), ischemia group (36.5 ± 0.5 ℃ after operation), 3h ischemia group and 3h ischemia group The rats in 1 h, 2 h, and 3 h groups were reperfused for 7 h after hypoxia. The hypothermia group (temporomandibular muscle temperature was controlled at 32.5 ± 0.5 ℃ after surgery) 2 h, 3h group each 7. The animal model is referenced to Zea longa’s model of middle cerebral artery occlusion and improved. Results Compared with ischemia group and hypothermia group, the production of glutamate (Glu), asparagine (Asp) and malondialdehyde (MDA) in ischemic cortex of hypothermia group decreased obviously, while superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) production and release increased significantly, γ-aminobutyric acid (GABA) content also increased. CONCLUSION: The changes of four kinds of amino acids and free radical system under mild hypothermia are important mechanisms for protecting ischemic neurons