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目的研究芪参复康胶囊对心理应激促发的高原低压低氧环境下大鼠肺损伤的干预效果。方法雄性SD大鼠随机分为低氧组,实验组(低氧+应激)和芪参复康胶囊干预组(低氧+应激+药物)。应激采用旁观电击心理应激模型。模拟高原低压低氧环境处置,把大鼠置于模拟海拔6000m低压舱内24h。用推挽灌流的方法收集海马细胞外液,用高效液相色谱-电化学测定海马细胞外液中的去甲肾上腺素(NE)的含量。通过测定大鼠肺湿/干比值、肺湿重/体重比值和与细支气管伴行小血管“袖套”面积/血管面积的比值来评定肺损伤程度。结果①实验组海马细胞外液中的NE含量、肺湿/干比值和血管“袖套”/血管比值均显著高于低氧组(t=2.453,2.514,2.813;P均<0.05);②干预组海马细胞外液中的NE含量、肺湿/干比值和血管“袖套”/血管比值均显著低于实验组(t=2.183,2.171,2.242;P均<0.05)。结论芪参复康胶囊能有效预防心理应激促发的低压低氧环境下的大鼠肺损伤。
Objective To investigate the intervention effect of Qishen Fukang capsule on lung injury induced by psychological stress in plateau hypobaric hypoxia environment. Methods Male SD rats were randomly divided into hypoxia group, experimental group (hypoxia + stress) and Qishen Fukang capsule intervention group (hypoxia + stress + drug). Stressed shock shock stress using the model. Simulated plateau hypobaric hypoxia environment disposal, the rats placed in a simulated altitude of 6000m low pressure cabin 24h. The extracellular fluid of hippocampus was collected by push-pull perfusion method and the content of norepinephrine (NE) in hippocampus extracellular fluid was determined by high performance liquid chromatography-electrochemistry. The degree of lung injury was assessed by measuring the ratio of wet / dry lungs, the ratio of wet weight to body weight, and the ratio of bronchiolar small vessels to cuff area / vascular area. Results ① In the experimental group, NE content, wet / dry lung ratio and vessel / cuff / blood vessel ratio in hippocampus were significantly higher than those in hypoxia group (t = 2.453, 2.514, 2.813, P <0.05) ; ②In the intervention group, NE content, wet / dry lung ratio and vessel / sleeve / blood vessel ratio in hippocampus were significantly lower than those in experimental group (t = 2.183,2.171,2.242, P <0.05). Conclusion QSFK can effectively prevent lung injury induced by psychological stress in rats under hypobaric hypoxia and hypoxia.