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目的研究人垂体腺瘤P16基因的改变,同时探索原位PCR技术的适宜条件和结果确认,以及用于基因缺失检测的可行性。方法原位PCR和免疫组化技术。结界绝大部分间质细胞为P16蛋白免疫组化阴性,而一部分垂体肿瘤细胞呈P16阳性;针对P16基因的原位PCR信号则可见于垂体肿瘤细胞和间质细胞等各种细胞,即P16组化阴性的细胞同样表明有P16基因存在。结论原位PCR可以是P16基因研究的一种有效手段,提示垂体腺癌的P16基因改变形式可能主要是表达过度而不是基因缺失。
Objective To study the alteration of P16 gene in human pituitary adenomas, and to explore the suitable conditions and results of in situ PCR, and the feasibility of gene deletion detection. Methods In situ PCR and immunohistochemistry. Most of the mesenchymal cells in the enchantment were negative for P16 protein immunohistochemistry, and some pituitary tumor cells were positive for P16; in situ PCR signals for the P16 gene were found in various cells such as pituitary tumor cells and mesenchymal cells, ie P16 The cells that were negative for histology also showed the presence of the P16 gene. Conclusion In situ PCR may be an effective method for the study of P16 gene, suggesting that the P16 gene in pituitary adenocarcinoma may be overexpressed rather than deleted.