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目的：探讨血清非免疫球蛋白成分 １０ ｋＤ蛋白与重症肌无力的关系。方法：（１）用 ＳＤＳ－ＰＡＧＥ分析健康人与 ＭＧ患者的血清蛋白。（２）用抗体测血清中 １０ ｋＤ蛋白水平。（３）观察 １０ ｋＤ蛋白对培养的健康人及 ＭＧ单核血细胞增殖的影响。结果：（１）１０ ｋＤ与 １４ ｋＤ扫描面积的比值存在明显差异，９７％的健康人面积之比＞１．而 ＭＧ患者仅有３０％。（２）１０ｋＤ蛋白浓度在健康人及ＭＧ患者两组间不存在有意义的差别。（３）１０ ｋＤ蛋白１００ μｇ／ｍｌ可抑制健康人未激活的和ＰＨＡ激活的单核血细胞增殖，但该蛋白和 ＰＨＡ对ＭＧ患者的细胞增殖无影响。结论：（１）血清 １０ｋＤ与 １４ ｋＤ蛋白扫描面积的比值可作为生化诊断 ＭＧ的辅助参数。（２）１０ ｋＤ蛋白具有调节健康人单核血细胞增殖的生物活性。
Objective: To investigate the relationship between serum non-immunoglobulin 10 kD protein and myasthenia gravis. Methods: (1) SDS-PAGE was used to analyze the serum proteins of healthy and MG patients. (2) 10 kD protein level in serum with antibody. (3) To observe the effect of 10 kD protein on proliferation of cultured monocytes and MG monocytes. Results: (1) There was a significant difference in the ratio of 10 kD to 14 kD scanning area, and 97% of healthy people area ratio> 1. While MG patients only 30%. (2) There is no significant difference between the two groups of 10kD protein concentration in healthy and MG patients. (3) 10 kD protein 100 μg / ml can inhibit the proliferation of uninactivated and PHA-activated mononuclear cells in healthy human, but the protein and PHA have no effect on the proliferation of MG patients. Conclusion: (1) The ratio of serum 10kD to 14 kD protein scan area can be used as assistant parameter for biochemical diagnosis of MG. (2) The 10 kD protein has the biological activity of regulating the proliferation of healthy human monocyte.