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目的探讨角膜移植排斥反应的发生机制及其参与细胞的表型。方法制备正常大鼠和穿透性角膜移植鼠的角膜和虹膜睫状体铺片，用８种单克隆抗体，于铺片上进行免疫组化染色。结果正常周边角膜及角膜缘可见少量的Ｔ细胞（ＣＤ３）、辅助／诱导性Ｔ细胞（ＣＤ４）、抑制／细胞毒Ｔ细胞（ＣＤ８）、巨噬细胞、树突细胞、主要组织相容性复合体Ｉ类抗原（ｍａｊｏｒｈｉｓｔｏｃｏｍｐａｔｉｂｉｌｉｔｙｃｏｍｐｌｅｘｃｌａｓⅡａｎｔｉｇｅｎ，ＭＨＣⅡ类抗原）阳性细胞及β转化生长因子（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒβ，ＴＧＦβ）阳性细胞；同种角膜移植术后７及１２天角膜和虹膜睫状体中有大量上述细胞，未见Ｂ细胞增多；自体角膜移植鼠未见此种反应。结论角膜移植排斥反应是一种多细胞反应，虹膜及睫状体的受累可能对排斥反应具有促进或加剧作用。
Objective To investigate the mechanism of corneal graft rejection and the phenotypes of its involved cells. Methods The corneal and iris ciliary body of normal rats and penetrating keratoplasty rats were prepared. Eight kinds of monoclonal antibodies were used to make immunohistochemical staining on the tablets. Results A small amount of T cells (CD3), helper / inducible T cells (CD4), suppressor / cytotoxic T cells (CD8), macrophages, dendritic cells and major histocompatibility complex The positive cells of major histocompatibility complex Ⅱ clasⅡantigen (MHC Ⅱ) and the cells of transforming growth factor β (TGF β) positive cells were found in corneal and iris ciliary body after 7 and 12 days A large number of these cells, no increase in B cells; autologous corneal transplantation rats did not see this response. Conclusion Corneal graft rejection is a multicellular reaction. The involvement of the iris and ciliary body may promote or exacerbate the rejection.