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本文研究了牛黄抗氧化作用及其原因,从分子水平上揭示这一传统中药的药理机制。 取体重250—270g重大白鼠,称重后断头处死,立即剖取肝脏,加生理盐水后匀浆。分别取肝匀浆1.5ml,牛黄生理盐水悬浊液0.1ml,混合后于37±1℃温育振荡1.5小时。离心后加20%三氯乙酸1.5ml,混
This paper studies the antioxidation of bezoar and its causes, and reveals the pharmacological mechanism of this traditional Chinese medicine at the molecular level. Take a weight of 250-270g of a large white rat, weighed and decapitated, and immediately dissect the liver and homogenate after adding normal saline. Separately, 1.5 ml of liver homogenate and 0.1 ml of bovine physiological saline suspension were collected, mixed, and incubated for shaking at 37±1° C. for 1.5 hours. After centrifugation, add 20% trichloroacetic acid 1.5ml, mix