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目的：观察适当浓度的ＥＢＶ－ＢＨＲＦ１重组蛋白（ｒＢＨＲＦ１）抽提液有无拮抗裸鼠脾细胞程序性死亡（ＰＣＤ）作用。方法：用１×１０６ｍｏｌ／Ｌ地塞米松作用１８ｈ、４３℃加热１０ｍｉｎ及无血清培养４８ｈ等方法诱导脾细胞发生ＰＣＤ，预先加入终浓度为２ｍｇ／Ｌ的ｒＢＨＲＦ１蛋白起拮抗作用。结果：未加ｒＢＨＲＦ１的对照组细胞发生典型的ＰＣＤ，即细胞ＤＮＡ的电泳出现ＰＣＤ特征性的梯形（ｌａｄｅｒ）ＤＮＡ降解片段；电镜观察显示核凝缩，但细胞膜及细胞器结构基本保持正常。若与适当浓度的ｒＢＨＲＦ１预培养８ｈ，再用上述方法处理，则因有重组蛋白的保护而未出现ＤＮＡ降解片段及ＰＣＤ的形态学变化。结论：初步显示ｒＢＨＲＦ１具有抑制脾细胞发生ＰＣＤ的功能。
OBJECTIVE: To observe the effect of EBV-BHRF1 recombinant protein (rBHRF1) extract on the apoptosis of splenocytes in nude mice. Methods: PCD was induced in spleen cells by 1 × 106mol / L dexamethasone for 18h, heating at 43 ℃ for 10min and serumserifying for 48h. The rBHRF1 protein with a final concentration of 2mg / L was antagonized. Results: The typical PCD cells were found in the control group without rBHRF1, that is, the degraded fragments of PCD characteristic of PCD appeared in the cell DNA electrophoresis. The electron microscope showed nuclear condensation but the cell membrane and organelle structure remained normal. If pretreated with appropriate concentration of rBHRF1 for 8h and then treated with the above method, the morphological changes of DNA degrading fragments and PCD did not occur due to the protection of the recombinant protein. Conclusion: The preliminary results show that rBHRF1 can inhibit the function of PCD in splenocytes.