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目的总结大鼠盲肠结扎穿刺法脓毒血症模型的制作和成功标志,探讨依达拉奉联合川芎嗪对脓毒血症大鼠肝功能的保护作用。方法SD大鼠40只分正常组、假手术组、手术对照组、依达拉奉联合川芎嗪治疗组(下称治疗组),每组10只。手术对照组和治疗组予结扎盲肠中部,用16号针头贯通穿孔,制作大鼠脓毒血症模型;治疗组于术前15m in及术后3h各联合腹腔注射依达拉奉5mg/kg和川芎嗪15mg/kg,术后18h采血;假手术组和手术对照组均进行腹水及心脏采血培养,血标本做丙氨酸氨基转移酶(ALT)、天冬门氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)检测。结果模型组腹腔见大量黄色脓性腹水或血性腹水,盲肠变黑、胀气伴恶臭味,手术对照组血培养1只阳性,腹水培养全部阳性,细菌鉴定为大肠杆菌、铜绿假单胞菌、梭形芽胞菌,多为混合感染。正常组、假手术组和手术对照组AST逐渐升高,差异均有统计学意义,假手术组和手术对照组AST比正常组高,P<0.01;治疗组AST较手术对照组明显下降,P=0.038。手术后(包括假手术)大鼠血ALT>100 IU/L,脓毒血症模型成功达91%,AST<100 IU/L者均无脓毒血症表现,P<0.01。结论盲肠结扎穿刺法是比较理想的脓毒血症模型,但不同来源的大鼠要采用不同的操作方式,进行预试,以确定最佳的穿刺孔径。大鼠病态表现、腹腔脓性或血性腹水、腹水细菌培养阳性是模型成功的标志,血ALT>100 IU/L亦可作为模型成功的判断指标。依达拉奉和川芎嗪联合应用能改善脓毒血症大鼠的肝功能。
OBJECTIVE: To summarize the production and success of sepsis model of cecal ligation and puncture in rats and explore the protective effect of edaravone and ligustrazine on liver function in septic rats. Methods 40 SD rats were divided into normal group, sham operation group, operation control group, edaravone combined with ligustrazine group (hereinafter referred to as treatment group), 10 rats in each group. The control group and the treatment group were ligated to the middle part of the cecum, and the rat sepsis model was made by perforating a 16-gauge needle. In the treatment group, edaravone 5mg / kg and 15mg / kg Ligustrazine 15mg / kg and blood 18h after operation. The sham-operated group and operation control group were cultured with blood and ascites fluid. ALT, AST, , Lactate dehydrogenase (LDH) test. Results In the model group, a large amount of yellow purulent ascites or bloody ascites were found in the abdominal cavity of the model group. The cecum was dark and flatulence was accompanied with malodour. One blood culture positive control group and all ascites culture positive were found in the control group. The bacteria were identified as Escherichia coli, Pseudomonas aeruginosa, Fusarium, mostly mixed infection. AST in normal group, sham operation group and operation control group increased gradually, the differences were statistically significant, AST in sham group and operation control group were higher than normal group, P <0.01; AST in treatment group was significantly lower than that in control group, P = 0.038. Blood ALT> 100 IU / L in rats after operation (including sham operation), sepsis model successfully reached 91%, AST <100 IU / L showed no sepsis, P <0.01. Conclusion Cecal ligation and puncture is an ideal model of sepsis, but different sources of rats to use different modes of operation, pre-test to determine the best puncture diameter. The pathological manifestations of rats, abdominal purulent or bloody ascites, ascites bacterial culture positive markers of success of the model, blood ALT> 100 IU / L can also be used as a model to determine the success of indicators. The combination of edaravone and ligustrazine can improve liver function in septic rats.