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目的:观察人参多糖注射液对人肝癌细胞BEL-7402和HpeG2增殖抑制作用,并探讨其机制。方法:将不同浓度的人参多糖注射液与人肝癌细胞BEL-7402和HpeG2作用,采用cell counting kit-8(CCK8)检测细胞的增殖抑制,通过倒置显微镜观察用药前后细胞形态、数量变化,透射电镜观察细胞坏死、凋亡等形态学变化,蛋白免疫印迹法(Western blot)检测肿瘤坏死因子受体1(TNFR1)蛋白表达情况。结果:人参多糖注射液对人肝癌细胞BEL-7402和HpeG2的增殖有明显抑制作用(P<0.05),并且与浓度和时间呈正相关。60,80,100 g·L~(-1)人参多糖注射液作用于人肝癌细胞BEL-7402和HpeG2 24,48,72h后,可显著抑制其增殖(P<0.01),40 g·L~(-1)人参多糖注射液作用于人肝癌细胞BEL-7402和HpeG2 24,48,72 h后可明显抑制其增殖(P<0.05)。人参多糖注射液作用人肝癌细胞BEL-7402和HpeG2 48 h后,细胞出现细胞核边集、凋亡小体等凋亡形态变化,72 h后部分细胞出现空泡样变等形态学变化。与空白组比较,40,60,80 g·L-1人参多糖注射液组作用人肝癌细胞BEL-7402和HpeG2后,TNFR1蛋白相对表达量明显升高(P<0.05,P<0.01)。结论:人参多糖注射液对人肝癌细胞BEL-7402和HpeG2的增殖有明显的抑制作用;诱导细胞凋亡可能是抑制作用的机制。
Objective: To observe the inhibitory effect of ginseng polysaccharide injection on the proliferation of human hepatocellular carcinoma cells BEL-7402 and HpeG2 and to explore its mechanism. Methods: Different concentration of ginseng polysaccharide injection was used to effect human hepatocellular carcinoma cells BEL-7402 and HpeG2. Cell counting kit-8 (CCK8) was used to detect the cell proliferation inhibition. The morphology and quantity of cells were observed by inverted microscope before and after treatment. Morphological changes such as cell necrosis and apoptosis were observed. The protein expression of tumor necrosis factor receptor 1 (TNFR1) was detected by Western blot. Results: Ginseng polysaccharide injection significantly inhibited the proliferation of human hepatocellular carcinoma cells BEL-7402 and HpeG2 (P <0.05), and was positively correlated with the concentration and time. The effect of 60, 80, 100 g · L -1 ginseng polysaccharide injection on human hepatocellular carcinoma cells BEL-7402 and HpeG2 24 h, 48 h and 72 h significantly inhibited the proliferation (P <0.01), 40 g · L ~ (-1) 1) Ginsenoside injection significantly inhibited the proliferation of human hepatocellular carcinoma cells BEL-7402 and HpeG2 at 24, 48 and 72 h (P <0.05). After the cells were treated with GEL for 48 h and 48 h, the morphology of apoptotic cells such as nucleus margins and apoptotic bodies were observed. After 72 h, morphological changes of some cells such as vacuolar degeneration were observed. Compared with the blank group, the relative expression of TNFR1 protein in 40, 60, 80 g · L-1 ginseng polysaccharide injection group was significantly increased (P <0.05, P <0.01) after treated with BEL-7402 and HpeG2. Conclusion: Ginseng polysaccharide injection significantly inhibits the proliferation of human hepatocellular carcinoma cells BEL-7402 and HpeG2; inducing apoptosis may be the mechanism of inhibition.