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目的 探讨脑缺血再灌注模型大鼠血脑屏障是否对高分子量的多肽 -碱性成纤维细胞生长因子 (b FGF)通透。方法 采用大脑中动脉腔内阻塞模型 ,股静脉滴注 1 2 5I- b FGF,对大脑冠状冰冻切片进行放射自显影 ,图象分析测定光密度。结果 正常对照大鼠脑冠状切片放射自显影结果显示脑轮廓影 ,且为全脑均匀分布 ;模型鼠除了显示脑轮廓影外 ,可见缺血病灶侧影像较健侧及正常鼠浓集。光密度测定结果显示模型鼠病灶侧与其健侧及正常鼠同侧的光密度值差异显著 (t=3.54,P<0 .0 5;F=39.6,P<0 .0 5)。结论 1 2 5I- b FGF经股静脉滴注后 ,能通过脑缺血再灌流模型大鼠血脑屏障并浓集于缺血脑区
Objective To investigate whether the blood-brain barrier (BBB) of rat model of cerebral ischemia-reperfusion can penetrate the high molecular weight human fibroblast growth factor (b FGF). Methods The middle cerebral artery occlusion model was established. The femoral vein was infused with 125 I-b FGF to autoradiograph the coronary frozen section of the brain. The optical density was measured by image analysis. Results The results of autoradiography in coronal sections of normal control rats showed cerebral silhouette and uniform distribution of the whole brain. In addition to the brain contour, the model rats showed thicker side images than the contralateral and normal rats. The results of optical density measurement showed that there was significant difference (P> 0.05) between the lesion side and the ipsilateral and normal side of model mice (P <0.05; F = 39.6, P <0.05). CONCLUSIONS: After 125I-bFGF is injected intravenously into the femoral vein, it can pass through the blood-brain barrier of cerebral ischemia-reperfusion model rats and accumulate in the ischemic brain area