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作者应用原位核酸杂交技术,检测了陕西籍不同组织学类型的鼻咽癌(NPC)_(60)例和鼻咽粘膜慢性炎症患者30例活检组织中的EB病毒DNA(EBV-DNA),并用鼻咽外肿瘤(肝癌、胃癌)40例进行对照观察。同时,对上述NPC中的12例低分化鳞癌进行血清抗体检测。核酸杂交结果:NPC60例中,高分化鳞癌、低分化鳞癌及未分化癌的EBV-DNA杂交阳性率分别为30％,73％,90％。经x~2检验,高分化鳞癌与后两者之间差异显著(P<0.001);鼻咽粘膜慢性炎症30例中3例呈EBV-DNA阳性;鼻咽外肿瘤未见EBV-DNA阳件。EBV抗体检测:低分化鳞癌12例中EBV壳抗原免疫球蛋白A抗体(EBV-VCA-IgA)阳性者11例(91％),其中9例(75％)EBV-DNA杂交阳性,两种检测结果一致。本实验结果表明:陕西地区NPC的发生中EBV感染因素可能具有病因学意义。
The authors used in situ hybridization to detect Epstein-Barr virus DNA (EBV-DNA) in Shaanxi NPC patients with different histological types of NPC (30 cases) and nasopharyngeal mucosa chronic inflammation patients (30 cases) And with nasopharyngeal tumors (liver cancer, gastric cancer) 40 cases were observed. At the same time, 12 cases of poorly differentiated squamous cell carcinoma were tested for serum antibody. Nucleic acid hybridization results: NPC6060 cases, well-differentiated squamous cell carcinoma, poorly differentiated squamous cell carcinoma and undifferentiated EBV-DNA hybridization positive rates were 30%, 73%, 90%. There was significant difference between the two groups (P <0.001). The EBV-DNA positive was found in 30 cases of chronic inflammation of the nasopharyngeal mucosa and EBV-DNA was not found in the nasopharyngeal carcinoma Pieces. EBV antibody detection: EBV shell antigen immunoglobulin A antibody (EBV-VCA-IgA) positive in 12 cases of poorly differentiated squamous cell carcinoma in 11 cases (91%), of which 9 cases (75%) EBV-DNA hybrid positive, The test results are consistent. The experimental results show that: EBV infection in NPC occurrence in Shaanxi Province may have etiological significance.