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目的评价不同频率丘脑底核(subthalamic nucleus,STN)电刺激对癫痫大鼠模型的抗癫痫效果。方法实验大鼠分为四组,记录电极置于双侧额叶及海马。在三组电刺激组中,我们使用了三种不同的频率,分别为30、130和260Hz。用立体定向方法将刺激电极置入至双侧丘脑底核。在对照组中,同样方法置入刺激电极,但不进行电刺激。电刺激30分钟后,腹腔注射海人酸诱导急性边缘叶发作,对脑电和发作强度进行评价。结果130和260Hz高频电刺激明显减少了脑电大发作的时间,其中以130Hz为著,但发作的总时间没有改变。130和260Hz高频电刺激延长了局部放电的时间,这可能是因为电刺激抑制了继发的大发作的结果。此外,这两种频率的刺激降低了发作的程度,延长了第一次发作的潜伏期,但是,30Hz频率的刺激无上述效果。结论基底节系统参与大发作的控制,表明高频电刺激可被用于某些发作形式的控制。
Objective To evaluate the anti-epileptic effects of electrical stimulation of subthalamic nucleus (STN) at different frequencies on epileptic rats. Methods The experimental rats were divided into four groups. The recording electrodes were placed on bilateral frontal lobe and hippocampus. In the three groups of stimulation groups, we used three different frequencies, 30, 130 and 260 Hz, respectively. Stimulation electrodes were implanted into the bilateral subthalamic nuclei using stereotactic methods. In the control group, the same method was used to implant the stimulation electrode, but no electrical stimulation was performed. Thirty minutes after electrical stimulation, intraperitoneal injection of kainate induced the onset of acute marginal lobe and evaluated the EEG and attack intensity. Results High-frequency electrical stimulation at 130 and 260 Hz significantly reduced the time of major electrocardiogram attack, with 130 Hz being the same, but the total duration of the attack did not change. High-frequency electrical stimulation at 130 and 260 Hz prolonged the time of partial discharge, probably because electrical stimulation inhibited the consequences of a secondary panic attack. In addition, the stimulation of these two frequencies reduced the degree of attack and prolonged the latency of the first attack, however, stimulation at 30 Hz did not achieve the above effect. Conclusions The basal ganglia system is involved in the control of a major episode, indicating that high-frequency electrical stimulation can be used for the control of certain seizures.