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目的研究硒化壳聚糖对急性早幼粒白血病细胞株NB4增殖和凋亡的影响,探讨这种影响与PML-RARα融合蛋白表达的关系。方法应用MTT法检测硒化壳聚糖对细胞增殖的影响,应用AO/EB荧光染色法共聚焦显微镜下观察诱导凋亡作用,应用Western blot的方法检测细胞PML-RARα融合蛋白含量的变化。结果硒化壳聚糖可对体外培养的NB4细胞产生剂量和时间依赖性的抑制作用,50 mg/L至100 mg/L硒化壳聚糖作用NB4细胞24 h可诱导细胞出现明显的凋亡现象,经硒化壳聚糖处理24 h后,NB4细胞内PML-RARα融合蛋白含量明显减少。结论硒化壳聚糖可通过下调PML-RARα融合蛋白含量对NB4细胞产生抑制增殖和诱导凋亡作用。
Objective To study the effect of selenized chitosan on the proliferation and apoptosis of acute promyelocytic leukemia NB4 cell line and to explore the relationship between the effect and the expression of PML-RARα fusion protein. Methods The effect of selenized chitosan on cell proliferation was detected by MTT assay. Apoptosis was observed by AO / EB staining under confocal microscope. The content of PML-RARα fusion protein was detected by Western blot. Results Selenized chitosan could inhibit the proliferation of NB4 cells in vitro in a time-and dose-dependent manner. NB4 cells induced by chitosan at a concentration of 50 mg / L to 100 mg / L could induce apoptosis obviously Phenol, the content of PML-RARα fusion protein in NB4 cells decreased significantly after treated with selenized chitosan for 24 h. Conclusion Selenium chitosan can inhibit the proliferation and induce the apoptosis of NB4 cells by down-regulating the content of PML-RARα fusion protein.