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目的了解多药耐药基因（ＭＤＲ１）和多药耐药相关蛋白基因（ＭＲＰ）在贲门癌组织中的表达及其临床意义。方法采用反转录聚合酶链反应（ＲＴ－ＰＣＲ）技术，检测了４６例贲门癌及癌旁组织中ＭＤＲ１和ＭＲＰ６４表达。结果癌组织中ＭＤＲ１和ＭＲＰ表达的阳性率分别６３％和５０％，均高于癌旁组织（Ｐ＜０．０５）；术前化疗的ＭＤＲ１ｍＲＮＡ和ＭＲＰｍＲＮＡ表达水平高于未化疗者（Ｐ＜０．０５）；中低分化肿瘤的ＭＤＲ１ｍＲＮＡ和ＭＲＰｍＲＮＡ表达水平高于高分化肿瘤（Ｐ＜０．０５）；结论贲门癌组织中具有内源和获得性多药耐药性；ＭＤＲ１和ＭＲＰ表达与贲门癌的ＴＮＭ分期无关，其高表达状态可预示肿瘤组织的分化不良。
Objective To investigate the expression and clinical significance of multidrug resistance gene (MDR1) and multidrug resistance-associated protein (MRP) in cardiac cancer. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of MDR1 and MRP64 in 46 cases of cardiac cancer and adjacent tissues. Results The positive rates of MDR1 and MRP expression in cancer tissues were 63% and 50%, respectively, which were higher than those in adjacent tissues (P<0.05). The expression of MDR1 mRNA and MRP mRNA in preoperative chemotherapy was higher than those in patients without chemotherapy (P<0). .05); MDR1 mRNA and MRP mRNA expression levels in moderately and poorly differentiated tumors were higher than those in well-differentiated tumors (P<0.05); Conclusions Endogenous and acquired multidrug resistance in cardiac cancer tissues; MDR1 and MRP expression and cardiac TNM staging of cancer is irrelevant, and its high expression status can predict poorly differentiated tumor tissue.