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目的 探讨用人源 M2三联体靶抗原检测 M2抗体在原发性胆汁性肝硬化诊断中的应用价值。方法间接免疫荧光法检测肝病患者,自身免疫病患者和健康体检者血清中的抗线粒体抗体。以人源M2三联体靶抗原建立的ELISA法与以猪心肌纯化组分作为抗原的免疫印迹法商业试剂盒检测M2抗体。结果20例原发性胆汁性肝硬化（PBC）均检出M2抗体,28例不明原因肝病6例M2抗体阳性。ELISA法的敏感性和特异性优于经典的间接免疫荧光法和商业试剂盒。结论 以人源M2三联体靶抗原建立的ELISA法具有很高的特异性和敏感性,为原发性胆汁性肝硬化的诊断提供了简便、快速而有效的手段。
Objective To explore the value of detecting M2 antibody with human M2 triad target antigen in the diagnosis of primary biliary cirrhosis. Methods Indirect immunofluorescence was used to detect anti-mitochondrial antibodies in serum of patients with liver disease, autoimmune diseases and healthy subjects. The M2 antibody was detected by the ELISA method established by the human M2 triplet target antigen and the commercial kit using the immunostaining method with the porcine myocardial purified component as an antigen. Results M2 antibodies were detected in 20 cases of primary biliary cirrhosis (PBC), and M2 antibodies in 28 cases of unexplained liver disease. The sensitivity and specificity of ELISA are superior to those of classical indirect immunofluorescence and commercial kits. Conclusion ELISA established by human M2 triplet target antigen has high specificity and sensitivity and provides a simple, rapid and effective method for the diagnosis of primary biliary cirrhosis.