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目的 探讨缺氧、缺血对新生鼠大脑组织天冬氨酸特异酶切的半胱氨酸蛋白酶 3(简称caspase 3)活性的影响及意义。方法 选择 7d龄Sprague Dawley大鼠 33只 ,结扎左侧颈总动脉后 ,吸入 8%氧气 2h ,制成缺氧、缺血性脑损伤 (HIBD)动物模型 ,HIBD后 0 5、12、2 4、48h取两侧大脑组织制成组织匀浆 ,用显色底物天冬氨酸 谷氨酸 缬氨酸 天冬氨酸 对硝基苯胺复合物测定caspase 3活性。结果 缺氧、缺血侧大脑组织caspase 3活性逐渐增高 ,2 4h后吸光度值达 1 0 0 47± 0 4390 ,48h后降至 0 45 6 9± 0 2 2 85 ,各时点间差异有极显著性 (P <0 0 0 1)。对侧大脑组织各时间点caspase 3活性未见明显变化 (P >0 0 5 )。结论 新生鼠大脑缺氧、缺血后caspase 3明显激活 ,支持细胞凋亡参与HIBD ,应用caspases抑制剂或其他抗凋亡药物 ,有可能为HIBD的治疗开辟新的途径
Objective To investigate the effect and significance of caspase 3 activity induced by aspartate-specific cleavage in neonatal rat brain tissue after hypoxia and ischemia. Methods Thirty-three Sprague Dawley rats aged 7 days were selected. After the left common carotid artery was ligated, 8% oxygen was inhaled for 2 hours to establish an animal model of hypoxic-ischemic brain injury (HIBD). After HIBD, , 48h to take both sides of the brain tissue made of tissue homogenate, with the chromogenic substrate aspartate glutamic acid valine aspartate p-nitroaniline complex measured caspase 3 activity. Results After hypoxia, the activity of caspase 3 in ischemic brain tissue gradually increased, the absorbance value reached 1047 ± 0 4390 after 24 hours and dropped to 0 45 6 9 ± 0 2 2 85 after 48 hours. There was a significant difference between the time points Significance (P <0 0 0 1). Contralateral brain tissue at each time point caspase 3 activity no significant change (P> 0 05). Conclusions Neonatal rat brain hypoxia, caspase 3 activation after ischemia, support for apoptosis in HIBD, the use of caspases inhibitors or other anti-apoptotic drugs may open up new avenues for the treatment of HIBD