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对新建的两种淋巴细胞性腹水瘤体外克隆,SAC和LAC-1细胞的分化状态检测,结果表明SAC及其未克隆的SRS细胞无T、B细胞标志,相当于淋巴干细胞;LAC-1细胞为Thy-1.1~+,无其他分化标志,相当于早期幼稚胸腺细胞。这两种瘤细胞的酸性α-醋酸萘酯酶(ANAE)反应主要呈M样。另外,用这两种瘤细胞对两个新单克隆抗体(C⑩,G(12))进行了辅助鉴定,还对正常小鼠不同成熟度的胸腺细胞和不同淋巴器官中淋巴细胞的ANAE作了检测。
For the newly established two kinds of lymphocytic ascites tumor clones in vitro, the differentiation status of SAC and LAC-1 cells, the results show that SAC and its uncloned SRS cells have no T, B cell markers, equivalent to lymphatic stem cells; LAC-1 cells For Thy-1.1~+, there is no other differentiation marker, equivalent to early naive thymocytes. The acid alpha-acetate naphthylase (ANAE) reaction of these two tumor cells was mainly M-like. In addition, two new monoclonal antibodies (C10, G(12)) were assisted in the identification of these two types of tumor cells, and the ANAEs of lymphocytes in different thymocytes and lymphoid organs of normal mice were also studied. Testing.