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为降解麻疯树籽饼粕中的毒性成分佛波酯,利用Klebsiella variicola进行固态发酵.采用单因素实验法研究了发酵温度、接种量、初始加水量、发酵时间对佛波酯降解率的影响.在此实验结果基础上,利用Design Expert8.0软件进行分析建立了发酵温度、接种量、初始加水量3个因素对佛波酯降解率影响的优化模型.该模型极显著(P<0.001),拟合度良好.经实验证实,在发酵温度为37℃、接种量33%(V/m)、初始加水量为105%(V/m)条件下发酵48 h,佛波酯降解率可达到84.30%,另一种毒性物质curcin的降解率可达63.21%.研究表明,利用响应面优化K.variicola固态发酵麻疯树籽饼粕过程,既可提高佛波酯降解率,也可降解curcin,具有生产指导意义.
In order to degrade phorbol ester, a toxic component of Jatropha curcas seed meal, solid state fermentation was conducted using Klebsiella variicola.The effects of fermentation temperature, inoculum size, initial water addition and fermentation time on the degradation rate of phorbol ester were studied by single factor experiment Based on the experimental results, the optimization model of the effect of phorbol ester on the degradation rate of phorbol ester was established by using Design Expert software 8.0, and the fermentation temperature, inoculum size and initial water addition amount were established.The model was very significant (P <0.001) , The fitting degree is good.Experimental results show that the degradation rate of phorbol ester can be fermented at the temperature of 37 ℃, the inoculation amount of 33% (V / m) and the initial water addition rate of 105% (V / m) Reached 84.30% and the degradation rate of curcin, another toxic substance, reached 63.21% .Research shows that the use of response surface to optimize K.variicola solid state fermentation of Jatropha curcas seed meal can not only improve the degradation rate of phorbol ester, but also degradation Curcin, with production guidance.