论文部分内容阅读
目的检测愤怒情绪模型大鼠海马γ-氨基丁酸B受体两个亚基GABABR1、GABABR2和内向整流型钾通道(KIR)表达的变化,初步探讨白香丹胶囊对肝气逆证的中枢干预机制。方法大鼠随机分为正常对照组、模型组、白香丹胶囊组(0.2 g·kg-1)、巴氯芬组(0.008 g·kg-1),采用“社会隔离结合居住入侵”方法制备愤怒情绪大鼠模型,药物组按相应剂量灌胃给药7 d,每日1次。通过糖水偏好实验、旷场实验和高架十字迷宫实验进行模型评价,用免疫荧光化学法检测各组大鼠海马GABABR1、GABABR2和KIR的表达。结果与正常对照组比较,模型组糖水偏好系数降低,旷场实验得分增高,高架十字迷宫实验进入开放臂次数百分数(OE%)和开放臂停留时间的百分数(OT%)降低,海马GABABR1、GABABR2和KIR表达水平降低P<0.05;与模型组比较,白香丹胶囊组和巴氯芬组旷场实验得分降低,OE%和OT%值升高,海马GABABR1、GABABR2和KIR表达水平升高(P<0.05)。结论大鼠愤怒情绪的产生可能与海马GABABR1、GABABR2和KIR表达降低有关,白香丹胶囊平肝理气的中枢机制可能与其激活海马GABABR及其G蛋白偶联的KIR有关。
Objective To investigate the changes of GABABR1, GABABR2 and inward rectifier potassium channel (KIR) in two subunits of γ-aminobutyric acid B receptor in hippocampus of rats with anorexia and to explore the central mechanism of Bai Xiang Dan Capsule . Methods The rats were randomly divided into normal control group, model group, Bai Xiang Dan Capsule group (0.2 g · kg -1) and baclofen group (0.008 g · kg -1), using “social isolation combined with residential invasion” Methods The rat model of anger was prepared, and the drug group was given intragastrically for 7 days at the corresponding dose, once a day. The model was evaluated by sweet water preference test, open field test and elevated plus maze test. The expression of GABABR1, GABABR2 and KIR in the hippocampus were detected by immunofluorescence staining. Results Compared with the normal control group, the preference coefficient of syrup in the model group decreased, the open field test scores increased, the percentage of open arms (OE%) and the open arms (OT%) in the elevated plus maze test decreased, while the levels of GABABR1, GABABR2 And KIR decreased significantly (P <0.05). Compared with model group, the scores of open field test, OE% and OT% in Bai Xiangdan capsule group and baclofen group increased, and the expression levels of GABABR1, GABABR2 and KIR in hippocampus increased P <0.05). Conclusions The generation of anger in rats may be related to the decreased expression of GABABR1, GABABR2 and KIR in the hippocampus. The central mechanism of Bai Xiang Dan Capsule Pinggan Qi may be related to its activation of GABABR and its G protein-coupled KIR.