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为了获得温室条件下条形柄锈菌发生体细胞重组而导致毒性变异的直接证据,本研究选取7个美国条形柄锈菌小麦专化型菌系和2个美国条形柄锈菌大麦专化型菌系按照夏孢子颜色和专化型与毒性差异组成9对菌系组合,对于室内混合接种产生的子代菌系用具有不同抗性的小麦或大麦品种进行筛选,采用毒性分析及SSR分子标记技术对条形柄锈菌体细胞重组现象进行了研究。对获取的413个单孢子代菌系进行的毒性分析结果显示,有84个单孢子代菌系的毒性谱表现与亲本菌系不同,初步证明体细胞重组过程的存在。SSR标记分析结果显示,11对SSR引物中有6对引物在5对菌系组合的28个毒性谱不同的单孢子代菌系中,检测发现3个单孢菌系的扩增条带与其亲本菌系不同,且表现为亲本菌系扩增条带的重组,为体细胞重组菌系。这一结果从分子水平上证明了条形柄锈菌在室内接种条件下可以通过体细胞重组产生新小种而导致毒性变异。
In order to obtain direct evidence of cytotoxicity caused by somatic cell recombination under the greenhouse conditions, seven S. typhimurium wheat-specific strains and two S. typhimurium barley cultivars According to the difference of uredospores color and specialization type, Toxoplasma gondii strain was composed of 9 pairs of bacterial strains, and the hybrid strains of wheat or barley with different resistance were screened for indoor hybrid inoculation. Toxicity analysis and SSR The molecular marker technique was used to study the somatic cell recombination phenomenon of P. striiformis. Toxicity analysis of 413 isolates of single spore germ obtained showed that the virulence spectrum of 84 isolates of monosporidiosis was different from that of the parental strain, which initially proved the existence of somatic cell recombination process. SSR marker analysis showed that among the 11 pairs of SSR primers, 6 pairs of primers were found in 28 strains of single spore germ strains with different toxicity profiles of 5 strains of bacteria. The amplification bands of 3 strains of Monospora and their parents were detected Different strains, and the performance of the amplification of the parental strains of recombinant bands for somatic recombinant strains. This result proves at the molecular level that P. tabaci can cause toxicity variation by somatic recombination of new races under in-house conditions.