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目的:探讨肠道缺血-再灌注损伤对不同肠段细菌易位的影响。方法:将大鼠随机分为对照组(行假手术)和肠道缺血-再灌注组(分别于再灌注后1、6和24 h取材)。检测空肠、回肠和结肠的细菌、内毒素易位情况,各段肠系膜静脉中的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。结果:对照组和肠道缺血-再灌注1 h组各肠段系膜淋巴结细菌培养均阴性,再灌注后24 h组空肠、回肠系膜淋巴结细菌易位率明显高于对照组和结肠系膜淋巴结(P<0.05);两组大鼠各段肠系膜静脉血培养、内毒素测定均为阴性;肠道缺血-再灌注后1 h和6 h组大鼠TNF-α浓度明显高于对照组(P<0.05);各段肠系膜静脉血IL-6浓度均明显高于对照组(P<0.05),再灌注后6 h组,空肠、回肠的肠系膜静脉血IL-6浓度明显高于结肠(P<0.05)。结论:肠道缺血-再灌注损伤可造成肠道细菌易位,空、回肠更易遭受肠缺血-再灌注损伤的打击,从而发生细菌易位。
Objective: To investigate the effect of intestinal ischemia-reperfusion injury on bacterial translocation in different intestinal segments. Methods: The rats were randomly divided into control group (sham operation) and intestinal ischemia-reperfusion group (taken at 1, 6 and 24 h after reperfusion). The bacteria in the jejunum, ileum and colon, the endotoxin translocation and the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in each stage of mesenteric vein were detected. Results: The bacterial culture of the intestinal segments of the intestinal segments in the control group and the intestinal ischemia-reperfusion 1 h group was negative. The bacterial translocation rate of the jejunum and ileum in 24 h after reperfusion was significantly higher than that in the control group and the mesenteric lymph node (P <0.05). Mesenteric venous blood culture and endotoxin test of each group were negative in both groups. The concentration of TNF-α in rats in 1 h and 6 h groups after intestinal ischemia-reperfusion was significantly higher than that in control group (P <0.05). The concentration of IL-6 in mesenteric venous blood in each stage was significantly higher than that in control group (P <0.05). The concentration of IL-6 in mesentery venous blood in jejunum and ileum at 6 h after reperfusion was significantly higher than that in colon <0.05). CONCLUSIONS: Intestinal ischemia-reperfusion injury can cause intestinal bacterial translocation, empty and ileum more vulnerable to intestinal ischemia-reperfusion injury, resulting in bacterial translocation.