哥斯达黎加管圆线虫虫卵在肠道组织内发育和孵化,是腹部管圆线虫病的致病因素。虫卵的体外培养系统对于研究哥斯达黎加管圆线虫病理学、生理学、生物学以及免疫学等提供了另一种工具。本文作者介绍了用已知成分的培养基成功地将虫卵培养至第一期幼虫的方法。 成虫取自实验感染2—4个月的棉鼠肠 The Costa Rican pipe worm, nematode, develops and hatches in the gut tissue and is a causative factor of the abdominal pipe worm nematode. The in vitro culture system of eggs provides an alternative tool for studying the pathology, physiology, biology and immunology of A. elegans in Costa Rica. The authors describe a method of successfully culturing the eggs to the first larvae using media of known composition. Adults were harvested from cotton rat intestine 2-4 months old