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目的观察人工消化法检验旋毛虫的效果及对肌幼虫活性、感染性的影响。方法将小鼠感染100条肌幼虫后30 d剖杀,分别应用3组消化法检验,即:消化2 h组(磁力搅拌法)、消化12 h组、消化20 h组,每组取肉样10份,每份含300个囊包,消化后分别计数每份肉样的幼虫数;同时对幼虫活性进行观察。40只昆明小鼠随机分为对照组和3个消化组(消化2、12、20 h组),共4组,每组10只。对照组:每鼠感染100个囊包;消化组:将3组方法收集的幼虫分别感染小鼠,每鼠经口感染100条幼虫。感染后30 d剖杀,取膈肌压片镜检,并用磁力搅拌法收集幼虫、计数。结果消化2、12、20 h组的幼虫检出率分别为78.47%、76.73%、68.63%,死亡率分别为0.59%、4.60%、7.43%。3组收集的幼虫分别感染小鼠,30 d后剖杀,与对照组相比,消化2、12、20 h组的减虫率分别为60.56%、61.94%、73.07%。结论磁力搅拌法(消化2 h)在幼虫检出率、幼虫活性、幼虫感染性等方面均优于消化12、20 h实验组,为进行旋毛虫检验和动物实验,优先选择的方法。
Objective To observe the effect of artificial digestion on Trichinella spiralis and its effect on the activity and infectivity of muscle larvae. Methods The mice were infected with 100 muscle larvae and then killed 30 days later. Three groups were digested by digestion method, namely digestion for 2 hours (magnetic stirring method), digestion for 12 hours, digestion for 20 hours, 10, each containing 300 caps, digestion were counted after each meat-like larvae; at the same time the activity of larvae were observed. 40 Kunming mice were randomly divided into control group and three digestion groups (digestion 2,12,20 h group), a total of 4 groups of 10. Control group: 100 mice per mouse infected bag; digestion group: three groups of larvae were collected from mice were infected with each mouse infected with 100 larvae. Dissected 30 d after infection, microscopic examination of the diaphragm was taken, and larvae were collected by magnetic stirring and counted. Results The detection rates of larvae in 2, 12 and 20 h groups were 78.47%, 76.73% and 68.63%, respectively, with mortality rates of 0.59%, 4.60% and 7.43%, respectively. The larvae collected from the three groups were infected respectively and were sacrificed 30 days later. Compared with the control group, the worm reduction rates of the digested 2, 12 and 20 h groups were 60.56%, 61.94% and 73.07%, respectively. Conclusion Magnetic stirring (digestion 2 h) is superior to digestion in 12 and 20 h experimental groups in detection rate of larvae, larval activity and larvae infectivity. It is a preferred method for Trichinella spiralis test and animal experiment.