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目的观察参芪扶正注射液对脐血细胞因子诱导的杀伤细胞(CIK细胞)体外增殖及杀瘤活性的影响。方法用脐血单个核细胞,诱导分化CIK细胞,分为4组。细胞因子组加入γ干扰素(IFN-γ)、白细胞介素1α(IL-1α)、白细胞介素2(IL-2)、CD3单抗细胞因子;参芪扶正注射液组:加入参芪扶正注射液;细胞因子+参芪扶正注射液组:加入参芪扶正注射液﹑IFN-γ、IL-1α、IL-2、CD3单抗细胞因子;对照组只加培养液。培养12天后CIK细胞达到增殖高峰期,用流式细胞仪检测细胞表型和增殖能力,MTT法检测CIK细胞对K562细胞的杀伤作用,ELISA法检测CIK细胞分泌的细胞因子IL-2、IFN-γ、肿瘤坏死因子α(TNF-α)水平。结果对脐血CIK细胞体外增殖的影响,在培养12天时,参芪扶正注射液组与细胞因子组比较差异无统计学意义(P>0.05),细胞因子+参芪扶正注射液组与细胞因子组相比差异有统计学意义(P<0.01);CIK细胞对K562细胞的杀伤作用,各实验组与对照组相比差异有统计学意义(P<0.01),参芪扶正注射液组CIK细胞在12、16天时分泌IL-2、IFN-γ、TNF-α因子水平优于对照组(P<0.01)。结论参芪扶正注射液对脐血CIK细胞既有显著的增殖和杀瘤作用,又可促进脐血CIK细胞分泌IFN-γ、IL-2和TNF-α。
Objective To observe the effects of Shenqi Fuzheng Injection on proliferation and cytotoxicity of cord blood cytokine-induced killer cells (CIK cells) in vitro. Methods Umbilical cord blood mononuclear cells were used to induce CIK cells and divided into 4 groups. In the cytokines group, IFN-γ, IL-1α, IL-2 and CD3 monoclonal antibody were added into the cytokines group. Shenqi Fuzheng injection group: Shenqi Fuzheng Injection of cytokines + Shenqi Fuzheng injection group: adding Shenqi Fuzheng injection, IFN-γ, IL-1α, IL-2, CD3 monoclonal antibody cytokines; control group only with broth. After 12 days of culture, CIK cells reached the peak of proliferation, cell phenotype and proliferation were detected by flow cytometry, cytotoxicity of CIK cells to K562 cells was detected by MTT assay, cytokines IL-2 and IFN- γ, tumor necrosis factor α (TNF-α) levels. Results There was no significant difference in the proliferation of CIK cells from umbilical cord blood when cultured for 12 days (P> 0.05). The cytokine + Shenqi Fuzheng injection group and cytokines (P <0.01). The killing effect of CIK cells on K562 cells was significantly different between the experimental and control groups (P <0.01). CIK cells in the Shenqi Fuzheng injection group were significantly different The levels of IL-2, IFN-γ and TNF-α secreted at 12 and 16 days were better than those of the control group (P <0.01). Conclusion Shenqi Fuzheng injection can significantly promote the proliferation and tumorigenesis of cord blood CIK cells and promote the secretion of IFN-γ, IL-2 and TNF-α by cord blood CIK cells.