作者先前的研究证明,华钩藤 Uncariasinensis(Oliv.)Havil 水提取物(USE)及其酚类和生物碱对谷氨酸诱导的培养 Wistar大鼠小脑颗粒细胞(CGC_s)神经元死亡具抑制作用。现作者研究了该植物对 NO 介导的神经毒性的防护作用。将该植物钩茎切碎,用沸水提取3次,合并提取液冻干得提取物(USE)。USE 经 Di-aion HP-20柱层析,用水洗脱得 USE-W;用 The author’s previous studies demonstrated that the aqueous extract of Uncaria sinensis (Oliv.) Havil (USE) and its phenols and alkaloids inhibited glutamate-induced neuronal death in cerebellar granule cells (CGC_s) of Wistar rats. . The authors studied the protective effect of this plant on NO-mediated neurotoxicity. The plants were chopped up and extracted 3 times with boiling water, and the combined extracts were lyophilized to obtain extracts (USE). USE column chromatography on Di-aion HP-20, eluting with water to obtain USE-W;