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本文探讨了用荧光光度法检测二氢奎尼丁的方法及实验条件，在０．０５ｍｏｌ／ＬＨ２ＳＯ４、１％ＨＡＣ介质中最大激发波长２５０ｎｍ、３６０ｎｍ，最大发射波长４４４ｎｍ，量子产率为０．５５（在０．０５ｍｏｌ／ＬＨ２ＳＯ４介质中）、０．５４（在１％ＨＡＣ介质中）。线性范围０·０５ｎｇ／ｍｌ～５．０μｇ／ｍｌ。检出限量为０．０１ｎｇ／ｍｌ。本方法简便、快速、灵敏度高，适用于临床医学监测服药病人血浆及尿液中药含量。
In this paper, the method and experimental conditions for the determination of dihydroquinidine by fluorescence spectrophotometry were investigated. The maximum excitation wavelength was 250nm, 360nm and the maximum emission wavelength was 444nm in 0.05mol / LH 2 SO 4 and 1% HAC media. The quantum yield was 0.55 (In 0.05 mol / LH 2 SO 4 medium), 0.54 (in 1% HAC medium). Linear range of 0.05ng / ml ~ 5.0μg / ml. The detection limit is 0.01ng / ml. The method is simple, rapid and sensitive, and is suitable for clinical medicine monitoring of Chinese medicine content in plasma and urine of patients taking medicine.