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肺癌是最常见的恶性肿瘤之一 .肺腺癌很容易发生转移 .实验以 17例肺癌 (5例肺腺癌、1例腺鳞癌、7例肺鳞癌及 4例小细胞末分化癌 )的原发灶、癌旁正常组织及转移淋巴结为材料 ,采用PCR及非放射性标记的 RNA斑点杂交分析 L TR在基因组的存在及其表达情况 .研究结果发现 :LTR序列在 17例肺癌病人的正常组织及肿瘤组织的基因组中普遍存在 ,L TR致肺癌转移与其插入基因组中无关 ;L TR致肺癌转移与其表达增高有关 ,而且其高表达与小细胞肺癌的转移无关 ,而与非小细胞肺癌的转移密切相关 .提示 L TR参与了非小细胞肺癌的转移过程 .实验结果为从细胞系获得的结论提供了进一步的证据
Lung cancer is one of the most common malignancies. Lung adenocarcinoma is very likely to metastasize. Experiments included 17 cases of lung cancer (5 cases of lung adenocarcinoma, 1 case of adenosquamous carcinoma, 7 cases of lung squamous cell carcinoma, and 4 cases of terminal cell differentiation carcinoma). The primary tumors, adjacent normal tissues, and metastatic lymph nodes were used as material. The presence and expression of LTR in the genome were analyzed using PCR and non-radioactive RNA dot blot hybridization. The study found that LTR sequences were normal in 17 patients with lung cancer. In the genomes of tissues and tumor tissues, ubiquitous LTR-induced lung cancer metastasis has nothing to do with its insertion into the genome; LTR-induced lung cancer metastasis is associated with its increased expression, and its high expression has nothing to do with the metastasis of small cell lung cancer, but with non-small cell lung cancer Metastasis is closely related. It is suggested that LTR is involved in the metastatic process of non-small cell lung cancer. The experimental results provide further evidence for the conclusions obtained from cell lines.